Wednesday, June 22, 2011

Black Women #1 Concern The hTERT Gene for Breast Cancer

June 22, 2011 By Robert Graham Reporting Park Plaza Medical Center in Houston Texas is looking at the hTERT Gene for Breast Cancer -----< Business--Wire>

Hybrid GenConnect Genomics Project at its new location at Park Plaza Medical Center in Houston Texas is looking at the hTERT Gene for Breast Cancer


hTERT (human telomerase reverse transcriptase) gene is the rate-limiting determinant of telomerase reactivation. The GenConnect study aims to quantitatively measure the expression of hTERT mRNA in human breast cancer, adjacent non-cancerous tissue (ANCT) and benign breast lesions, examine the association between hTERT and the clinicopathological characteristics of the cancer specimens and to explore the relationship between c-Myc and hTERT expressions. GenConnect extracted RNA from 49 breast carcinomas, 46 matched ANCT, and eight fibroadenomas. hTERT and c-Myc mRNA expressions were estimated by reverse transcriptase-PCR (RT-PCR) and Taqman methodology.


Hybrid GenConnect Genomics R&D shows that Breast cancer is the second leading cause of cancer death among American women. African American (AA) women are more likely to die from breast cancer than Caucasian (CAU) women revealing a clear disparity between AA women and women of other ethnicities. Genetic Data collected by Hybrid GenConnect and others reveal that although AA women show a lower incidence of breast cancer than CAU women, AA women tend to have larger, more aggressive tumors, and have a lower 5-year survival rate than their CAU, Hispanic, Asian and Native American counterparts. Importantly, AA women are more likely to die from breast cancer than any other ethnic group. While socio-economic, cultural and environmental factors are thought to contribute to these disparities, little is known as to whether biological differences may exist between CAU and AA women with breast cancer. To determine whether biological factors contribute to differential cellular responses in AA and CAU women, triple-negative breast cancer cells from AA and CAU women were cultured and treated with dietary bioactive compounds (−)- epigallocatechin-3-gallate (EGCG) and sulforaphane (SFN), both of which are known to actively repress breast cancer. Preliminary findings reveal differential RNA expression of the human telomerase reverse transcriptase gene (hTERT) in AA and CAU cells. Additionally, AA cells showed differences in responsiveness to ECGG and SFN treatments when compared to CAU cells. These results support
the hypothesis that biological differences may contribute to the disparity among AA and CAU women with breast cancer.

Given Treatment of A431 cells with green tea polyphenols and its components, epigallocatechin-3-gallate, epigallocatechin, and epicatechin-3-gallate, resulted in the formation of internucleosomal DNA fragments, characteristic of apoptosis. Treatment with epigallocatechin- 3-gallate also resulted in apoptosis in HaCaT, L5178Y, and DU145 cells, but not in NHEK. Confocal microscopy and flow cytometry confirmed the findings. The DNA cell cycle analysis showed that in A431 cells, epigallocatechin- 3-gallate treatment resulted in arrest in the G0−G1 phase of the cell cycle and a dose-dependent apoptosis. Conclusions: Green tea may protect against cancer by causing cell cycle arrest and inducing apoptosis. It needs to be evaluated in human trials.
Given Treatment sulforaphane, a natural component of broccoli and other brassica vegetables, reduces the growth of breast cancer stem cells and impairs their viability. The effects of sulforaphane on breast cancer stem cells in the laboratory were evaluated using various methods. Sulforaphane was found to reduce the stem cell population (as measured by a stem cell marker) by 65% to 80% in human breast cancer cells. In an additional experiment, mice were implanted with breast cancer xenografts and the tumors were treated directly with sulforaphane. The impact of the treatment was assessed in part by measuring tumor growth upon cell reimplantation into a second group of mice. Daily injection with 50 mg/kg sulforaphane for two weeks was found to reduce the number of stem cells by more than 50% in the xenograft tumors. Sulforaphane's ability to eliminate breast cancer stem cells appeared to prevent tumor growth after the reimplantation of primary tumor cells into the second group of mice. The authors conclude that sulforaphane inhibits breast cancer stem cells and that the findings warrant further clinical evaluation.

Tuesday, June 14, 2011

Breast Cancer Survivors Families Epidemiology & Genomics Can Stop Cancer in its Tracks

June 14, 2011 By Robert Graham Reporting from University of Texas Medical Center Houston -----< Business--Wire>


Breast Cancer Survivors Families Epidemiology & Genomics can Stop Cancer in its Tracks


Epidemiology: The study of the patterns, causes, and control of disease in groups of people.
Genomics: The study of the complete genetic material, including genes and their functions, of an organism.

Cancer epidemiology seeks to understand the biological, environmental and social risk factors of cancer with the goal of guiding efforts at prevention and

treatment. Examples of cancer research by Hybrid GenConnect researchers includes evaluation of the efficacy of chemoprevention to prevent secondary tumors, the association between racial disparities in breast cancer survival, the relationship between insulin resistance and breast cancer incidence, and the identification of genetic cancer susceptibility risk factors
Although genetic influences on breast cancer risk have been recognized for many years, high-throughput genotyping involving genome-wide association studies has transformed research on the etiology of cancer and many other diseases. This technological breakthrough and the agnostic search for signals have opened up avenues for research that otherwise would have remained hidden for years. For instance, we may not know why a variant in a "gene desert" (a stretch of DNA devoid of protein-encoding genes) is consistently associated with risks of breast, prostate, and several other cancers, but we must infer that an important model of genes and cancer induction needs major revision
Hybrid GenConnect Inc Genetic research experts within the Breast Cancer Division are investigating the role of genes in such diseases as Alzheimer’s disease, cardiovascular disease, diabetes, hypertension, obesity, respiratory diseases and vision disorders. Understanding the genetics of these diseases involves locating and characterizing the underlying genes, characterizing the extent and utility of DNA variation within and among populations, determining how patterns of DNA variation evolved in time and space, and establishing the impact of gene variation on the health of individuals, families and populations. Hybrid researchers are also responsible for developing computational and bioinformatic approaches and resources for investigating the genetic etiology of disease.
Hybrid GenConnect Research in Genomic Epidemiology (CHARGE), which is led by a team at Park Plaza Hospital, hope that pooling their combined resources will help speed the process of defining all the genetic factors involved in Breast Cancer. “Our first efforts will be to bring together all the data from the different groups so that they can be analyzed,” for Drug Safety/Epidemiology.
The new biorepository facility offers a wide range of storage options, including automated carousel controlled-room temperature storage at 15oC to 27oC, bulk sample storage at 15oC to 27oC, walk-in cold sample storage -20oC to 5oC, ultra-low temperature storage -70C to -80C, and -190oC vapor phase liquid nitrogen. In addition, Hybrid GenConnect maintains FDA 21 CFR Part 11 validated technology systems and adheres to U.S. Food and Drug Administration regulations, as well as U.S. Department of Transportation and International Air Transport Association guidelines.
About Hybrid GenConnect Inc,.
For 23 years, Hybrid Medical eNotebook Inc Core Competency are Biotech Engineering, Software Engineers, and Enterprise Networking. At Hybrid Medical we pride ourselves on our Boutique Relationship with our Private Sector and Public Sector Clients. This methodology allows us to be able to offer our Clients “End to End” Solutions to their Enterprise Services. We have done extensive Research in Service Oriented Architecture (S.O.A) and Enterprise Service Bus (ESB) and we feel that our Strategies and your Strategies create valuable Synergies. Let's investigate the Possibilities for Synergies between our Company and yours.
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